Figure 5.

Depletion of DGCR8 and hRRP6 Specifically Stabilizes Mature snoRNAs

(A) Schematic representation of U16 snoRNA location in intron 3 of the host RPL4 pre-mRNA. BS, branch site; E4, exon 4.

(B) HeLa cells were transiently depleted of DGCR8, hRRP6, hDIS3, RBM7, and ZCCHC7, and the levels of mature U16 were quantitated by qRT-PCR, using primers depicted on top of the panel.

(C) Quantification of the host pre-mRNAs containing U16 snoRNA in HeLa cells depleted for all factors depicted in (B), but also including hRRP41. For levels of depletion in (B) and (C), see Figure S5D. All values represented in the two panels are the average of at least three biological replicas showing ± SEM. Asterisks denote significant p value (≤0.05) by Student’s t test.