Skip to main content
. Author manuscript; available in PMC: 2016 Dec 21.
Published in final edited form as: Dev Cell. 2015 Dec 21;35(6):789–802. doi: 10.1016/j.devcel.2015.11.015

Figure 1. Expansion of the mouse ventricular system following neural tube closure.

Figure 1

(A) Sox2-positive neuroepithelial cells (green) contact the AF before neural tube closure at E8.5 (left panel), and the nascent CSF following neural tube closure at E9.5 (middle panel) and E10.5 (right panel). Nuclei counterstained with Hoechst. Scale bars: 50μm (left panel); 200μm (middle, right panels). (B) Representative ultrasound images of developing mouse (E8.5-E12.5). Dashed white line (left panel) outlines embryo. Scale bar: 2mm. (C) 3D reconstruction of ventricular system from ultrasound images sequences captured in (B) from E9.5-E12.5 with the following color representation: turquoise: lateral ventricles; yellow: third ventricle and aqueduct; dark blue: fourth ventricle. (D) Quantification of ventricle size based on 3D reconstructions of ultrasound image sequences (B, C, data not shown; ventricular volume (μl) represented as mean ± SEM: Lateral ventricles: E9.5 = 0.09 ± 0.02; E10.5 = 0.77 ± 0.12; E11.5 = 0.83 ± 0.07; E12.5 = 1.39 ± 0.19; E13.5 = 1.08 ± 0.05; Third ventricle and aqueduct: E9.5 = 0.31 ± 0.03; E10.5 = 1.48 ± 0.37; E11.5 = 1.33 ± 0.15; E12.5 = 1.40 ± 0.11; E13.5 = 1.16 ± 0.03; Fourth ventricle: E9.5 = 0.54 ± 0.04; E10.5 = 1.63 ± 0.22; E11.5 = 1.36 ± 0.06; E12.5 = 1.27 ± 0.27; E13.5 = 0.50 ± 0.14; Total ventricular volume: E9.5 = 0.94 ± 0.04; E10.5 = 3.92 ± 0.70; E11.5 = 3.51 ± 0.16; E12.5 = 4.06 ± 0.06; E13.5 = 2.74 ± 0.09; n = 3; ANOVA statistical analyses of total ventricular volume represented as (*) p < 0.05 and (***) p < 0.0001; n=3).