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. Author manuscript; available in PMC: 2015 Dec 26.
Published in final edited form as: Science. 2015 Sep 17;350(6259):455–459. doi: 10.1126/science.aac7442

Figure 2. Ro60 binds an RNA motif derived from Alu elements.

Figure 2

(A) Top four RNA motifs(14) identified from the Ro60 iCLIP peaks shared between K562 and GM12878. T=U in RNA. (B) RIP-PCR for Alu or HY RNA in Ro60 or IgG immunoprecipitates across a time course of IFNα treatment in GM12878 cells. Error bars represent SD of 3 technical replicates, data shown is a representative experiment of 3 (C) EMSA of Alu RNA motif (oligo b from panel D) with indicated concentrations of recombinant Ro60 protein. (D) EMSA of Alu motif variants with recombinant Ro60. a, PolyU sequence alone; b, representative Alu motif; bas, antisense of b; c, deleted polyU; d, partial 5’ and 3’ truncations; e, partial 3’ truncation. *=RNA/Ro60 complex. RNA sequences depicted in bottom panel. See also Fig. S4.