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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Nanomedicine. 2015 Jan 14;11(3):559–567. doi: 10.1016/j.nano.2014.11.011

Figure 4. Filopodial actin and growth cone responses to elongation.

Figure 4

(a) Thirty minutes after elongating a Thy1-SPION-treated filopodium to a PDL- and laminin-coated electromagnet tip (asterisk), the RGC growth cone did not change its position. Expression of RFP-LifeAct peptide in RGCs allowed visualization of F-actin filaments inside the elongated filopodium (white arrows). (b) A filopodium was elongated using CtxB-SPION, and anchored to the PDL-/laminin-coated electromagnet tip (asterisk). Engorgement of the filopodium after 30 minutes was observed (black arrowhead). The originating segment of the filopodium relocated to a new position (black arrows) in response to mechanical tension. Scale bar 10 μm in a and b.