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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: J Nutr Biochem. 2015 Aug 20;27:16–26. doi: 10.1016/j.jnutbio.2015.08.012

Figure 1. Effect of Gln supplementation on chronic ethanol feeding in mice.

Figure 1

A & B: Adult mice were fed Lieber-DeCarli liquid diet with (● EF & ■ EF-Gln) or without (○ PF & □ PF-Gln) ethanol (1–6% over 4 weeks) and with (○ PF & ● EF) or without (□ PF-Gln & ■ EF-Gln) Gln supplementation. Animals in all groups were pair fed to control a similar diet intake in all groups. Diet intake was measured daily (A). Body weight of animals recorded twice a week (B). Values are mean ± SE (n = 6). C & D: Mice were fed a Gln-free amino acid diet, a modification of Lieber-DeCarli liquid diet with (● GFEF, ▲ GNEF & ■ GSEF) or without (○ GFPF, △ GNPF & □ GSPF) ethanol (1–6% over 4 weeks) and without (○ GFPF & ● GFEF) or with 8.4 g/L diet (△ GNPF & ▲ GNEF) or 16.8 g/L diet (□ GSPF & ■ GSEF) Gln supplementation. Animals in all groups were pair fed to control a similar diet intake in all groups. Diet intake was measured daily (A), and body weights recorded twice a week (B). Values are mean ± SE (n = 6). Asterisks indicate the values that are significantly (p<0.05) different from values for corresponding non-ethanol groups.