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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Alcohol. 2015 Oct 27;49(8):781–794. doi: 10.1016/j.alcohol.2015.08.006

Fig. 8. Ethanol antagonized the decreased sIPSC frequency induced by low-frequency afferent stimulation (LFS) in BLA slices.

Fig. 8

A, B) Low frequency electrical afferent stimulation (0.1 Hz) in BLA induced a decrease in sIPSC frequency only when the patch electrode was loaded with the standard CsCl-based intracellular solution plus the eCB AEA (AEA loaded), but not in the absence of the eCB (CsCl) (n = 3, 9). C, D) The decrease in sIPSC frequency induced by LFS in AEA-loaded neurons is antagonized by SR 1-μM bath application throughout the experiment (n = 7). E, F) The decrease in sIPSC frequency induced by LFS in AEA-loaded neurons is also prevented by bath application of 80-mM ethanol (n = 5) (all scale bars 50 pA, 5 sec).