Figure 4.

ERβ-specific inhibitor PHTPP blocks inhibition of cell proliferation by EGCG. MCF-7/BOS cells were pretreated with the ERα-specific inhibitor MPP (1 μM) or the ERβ-specific inhibitor PHTPP (1 μM) for 24 hours and then treated with 50 μM EGCG for an additional 72 hours. Cell numbers were determined by hemocytometer and expressed as a percentage of the DMSO control (set at 100%). Values are expressed as mean ± SE (n = 3). ^∗Significantly different compared to control, P < 0.05; ^§significantly different compared to treatment with EGCG alone, P < 0.05.