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. 2015 Dec 13;2016:5161248. doi: 10.1155/2016/5161248

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Copyright © 2016 Xin Chen et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Histomorphometric Analysis of Penile Tissue. (a) Representative images are presented for the nNOS-positive fibers (black arrow indicated) in the penile dorsal nerves of each experimental group via immunohistochemical analysis. Original magnification is ×400. n = 6 per group. (b) Quantitative analysis of the nNOS-positive fibers to the total area of penile dorsal nerves in pixels calculated by Image-Pro Plus 6.0 software at a magnification of ×400. ^∗ P < 0.05. (c) Representative images of the S100-positive fibers in the penile dorsal nerves of each experimental group via immunofluorescence analysis. Original magnification is ×400. n = 6 per group. (d) Quantitative analysis of the S100-positive fibers to the total area of penile dorsal nerves in pixels calculated by Image-Pro Plus 6.0 software at a magnification of ×400. ^∗ P < 0.05. (e) Representative images of the penile tissue stained with Masson's trichrome in all experimental groups. Smooth muscle is stained red, and collagen and connective tissue are stained blue. Original magnification is ×100, n = 6 per group. (f) The ratio of smooth muscle to collagen and connective tissue area in the corpus cavernosum was calculated in each group. ^∗ P < 0.05. nNOS = neuronal nitric oxide synthase.