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. Author manuscript; available in PMC: 2017 Jan 30.
Published in final edited form as: J Neurosci Methods. 2015 Nov 3;258:94–103. doi: 10.1016/j.jneumeth.2015.10.012

Figure H.

Figure H

Procedure to move ES- and PS-exposed mice during defeat sessions.

Emotional Stress (ES) mice are shown in blue, Physical Stress (PS) mice are shown in red, and CD1 aggressors are shown in white. Notice aggressors are on the right side of the divider in all cages. ES and PS mice are alternated (as shown in Initial Setup) in every other cage. To begin the defeat, PS mice are moved to the next cage to the right into the aggressor’s territorialized home cage (there should be an ES mouse on the other (left) side of the divider). After the defeat session, ES mice are moved to another cage to the left (still on the left side of the divider). The PS mice are moved over the divider (to the left) in the same cage (where the ES mice was previously). This ensures that the PS-exposed mice are defeated by different aggressors and sleep in the aggressor’s home cage overnight.