Fig. 2.

SNX15 regulates APP processing and Aβ generation. a N2a695 cells were transiently transfected with SNX15 and control vectors for 36 h, and secreted Aβ40 and Aβ42 in conditioned media were quantified by ELISA, n =5, *p<0.05. b N2a695 cells were transiently transfected with mouse SNX15 shRNA (shSNX15-1 and −2) and Control shRNA (Ctrl shRNA) for 72 h, and secreted Aβ40 and Aβ42 in conditioned media were quantified by ELISA, n =4, *p<0.05. c Equal amounts of conditioned media in (a and b) were subjected to western blot analysis for sAPPα and sAPPβ, and equal protein amounts of treated cell lysates were analyzed by western blot analysis for indicated proteins. d-f Levels of sAPPα (d), sAPPβ (e) and APP β-CTF (f) in (c) were quantified by densitometry, normalized to those of β-actin, and compared to those of controls (set as one arbitrary units), n=3, *p<0.05, **p<0.01. g, h Primary cultured APPswe/PSEN1dE9 mouse neurons were infected with AAV8 expressing SNX15 (AAV SNX15) or EGFP (as control, AAV Ctrl) for 72 h. g Secreted Aβ40 and Aβ42 in conditioned media were quantified by ELISA, n=3, *p<0.05, **p<0.01. h sAPPα in conditioned media and indicated proteins in cell lysates were subjected to western blot. sAPPα levels were quantified by densitometry for comparison, n=3, *p<0.05.