Table 2.
Phenolic compounds identified in L. sibiricus extracts using HPLC-ESI-MS/MS method.
| Peak number | Phenolic compounds | max (nm) | RT (min) | Molecular (precursor) ion [M-H]− (m/z) | Main fragment ions (m/z) |
|---|---|---|---|---|---|
| 1 | 4-Hydroxybenzoic acid | 211, 253 | 15.26 | 137 | 93 [M-CO2-H]− |
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| 2 | (+)-Catechin (flavan-3-ol) |
230, 278 | 15.90 | 289 | 245 [M-CH2-CH-OH-H]− |
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| 3 | Chlorogenic acid | 217, 241 294, 231 |
18.13 | 353 | 191 [QA-H]−; 179 [CA-H]−
173 [QA-H2O-H]−; 135 [CA-CO2-H]− |
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| |||||
| 4 | Caffeic acid | 215, 241 291, 319 |
18.81 | 179 | 135 [M-CO2-H]−; 107 [M-CO2-CO-H]−
89 [M-CO2-CO-H2O-H]− |
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| 5 | p-Coumaric acid | 207, 226 291, 307 |
22.67 | 163 | 119 [M-CO2-H]− |
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| 6 | Ferulic acid | 214, 239 291, 325 |
23.88 | 193 | 179 [M-CH2-H]−; 149 [M-CO2-H]−
135 [M-CO2-CH2-H]− |
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| 7 | Rutin | 255, 265 294, 352 |
27.89 | 609 | 301 [M-rutinose-H]− |
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| 8 | Quercetin | 253, 268 297, 368 |
33.58 | 301 | 271 (M-CH2O-H]−; 179 [M-Bring-H]−
151 [M-Bring-CO-H]− |
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| 9 | Verbascoside | 237, 296, 330 | 29.63 | 623 | 669 [M+HCOO]−; 461 [M-H-CA]−
161 [CA-H2O-H]− |
CA: caffeic acid; QA: quinic acid.