Figure 4.

Expression of S100 calcium binding protein A9 in positive and negative controls. (a) Positive controls (normal cervical squamous epithelium) where strong to moderate expression of S100A9 was limited to the differentiated prickle and functional layers with no immunostaining of the regenerative basal layer. Expression of S100A9 was cytoplasmic and nuclear. (b) Negative controls (normal cervical squamous epithelium) without the addition of the anti-S100A9 antibody. All the images were acquired at 200x magnification and 400x. Scale bars = 50 μm and 20 μm.