Figure 3.

Effects of dose (A) and time (B) on DHA-induced apoptosis in primary CLL cells (10 samples). CLL cells viability was assessed by Annexin V-FITC/7-AAD immunostaining after exposure to DHA at concentrations ranging from 0,1 µM to 100 µM for 24 hours (A) and after exposure to DHA 10 µM for 24, 48 and 72 hours (panel B). Viable cells assessed by % of both Annexine V and 7-AAD negative are shown as mean ± standard error of mean. AnnV-7AAD-, Annexine V-negative and 7AAD-negative; OA, oleic acid; DHA, docosahexaenoic acid.