Figure 2.

Cystic colony formation from dissociated fetal mouse pancreas cells. A: Phase contrast image showing that BMP-6 promotes colony formation. Open arrows indicate colonies ≤ 30 μm; B: Immunocytochemical analyses: a: Proinsulin staining. Fixed colonies were stained with proinsulin antibody (brown); b: Activin A antagonizes colony formation; c, d: Insulin staining. Histological sections of harvested colonies were stained with anti-insulin antibody (brown). Adapted and modified from ref.[14].