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. 2015 Dec 16;5(12):e009597. doi: 10.1136/bmjopen-2015-009597

Table 3.

Baseline physical examinations and laboratory tests, Bushehr Elderly Health Programme study

Item Method of measurement
Physical examination
 Arterial blood pressure Manually by standard mercury sphygmomanometer in sitting position
 Weight in kg Stadiometer; heavy outer garments will be removed
 Height in cm Stadiometer; shoes will be removed
 Waist circumference in cm At the midway level between the costal margins and the iliac crests
 Hip circumference in cm At the level of the greater trochanters
Laboratory tests
 CBC Automated haematology analyser
 Fasting blood sugar enzymatic (glucose oxidase) colourimetric method using a commercial kit (Pars Azmun Inc, Tehran, Iran)
 Lipid profile (total cholesterol, LDL-C, HDL-C, triglyceride) Serum total cholesterol and HDL cholesterol will be measured using a cholesterol oxidase phenol aminoantipyrine and triglycerides using a glycerol-3-phosphate oxidase phenol aminoantipyrine enzymatic method. Serum LDL cholesterol will be calculated using the Friedwald formula; LDL cholesterol will not be calculated when triglycerides concentration is >400 mg/dL
Procedures
 Electrocardiography 12-lead, by a trained nurse
 Echocardiography Using M-Turbo Ultrasound System, Manufactured by SonoSite, Inc, by a cardiologist

CBC, complete blood count; HDL, high-density lipoprotein; LDL, low-density lipoprotein.