Table 3.
Item | Method of measurement | |
---|---|---|
Physical examination | ||
Arterial blood pressure | Manually by standard mercury sphygmomanometer in sitting position | |
Weight in kg | Stadiometer; heavy outer garments will be removed | |
Height in cm | Stadiometer; shoes will be removed | |
Waist circumference in cm | At the midway level between the costal margins and the iliac crests | |
Hip circumference in cm | At the level of the greater trochanters | |
Laboratory tests | ||
CBC | Automated haematology analyser | |
Fasting blood sugar | enzymatic (glucose oxidase) colourimetric method using a commercial kit (Pars Azmun Inc, Tehran, Iran) | |
Lipid profile (total cholesterol, LDL-C, HDL-C, triglyceride) | Serum total cholesterol and HDL cholesterol will be measured using a cholesterol oxidase phenol aminoantipyrine and triglycerides using a glycerol-3-phosphate oxidase phenol aminoantipyrine enzymatic method. Serum LDL cholesterol will be calculated using the Friedwald formula; LDL cholesterol will not be calculated when triglycerides concentration is >400 mg/dL | |
Procedures | ||
Electrocardiography | 12-lead, by a trained nurse | |
Echocardiography | Using M-Turbo Ultrasound System, Manufactured by SonoSite, Inc, by a cardiologist |
CBC, complete blood count; HDL, high-density lipoprotein; LDL, low-density lipoprotein.