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. 2016 Jan;22(1):87–95. doi: 10.1261/rna.052894.115

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© 2015 Wang et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 4. — Suppression of PP2A by miR-3661 increases its target phosphorylation in human cells. (A) Schematic overview of pri-miR-3661 expression plasmids. (B) The RNA levels of Hsa-miR-3661 were measured from 293FT cells transfected with the control vector and two constructs of pri-miR-3661 expression plasmids. (C) The mRNA levels of PPP2CA and PPP2CA-iso were measured from 293FT cells transfected with the control vector and two constructs of pri-miR-3661 expression plasmids. (D) The protein levels of PP2Acα, phosphorylated AKT (p-AKT), AKT, phosphorylated GSK-3β (p-GSK-3β), and GSK-3β were measured from 293FT cells transfected with the control vector and two constructs of pri-miR-3661 expression plasmids.