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. 2015 Nov 15;29(22):2331–2336. doi: 10.1101/gad.269324.115

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© 2015 Ye et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 3. — Sestrin2 is necessary for mTORC1 repression during AAD. (A) Immunoblots of lysates from wild-type and Sesn2^−/− MEFs cultured in leucine-free medium for up to 24 h. (B) Immunoblots of lysates from wild-type and Sesn2^−/− MEFs cultured in amino acid-free media as indicated for 24 h. (C) Immunofluorescence against mTOR and the lysosomal marker LAMP2 in wild-type, Gcn2^−/−, and Sesn2^−/− MEFs cultured in ±leucine medium for 24 h. Bars, 10 µm.