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. 2015 Nov 15;29(22):2391–2404. doi: 10.1101/gad.269050.115

Figure 4.

Figure 4.

The ChAS1 and PAR/ChCUC pathways converge on ChBP regulation. (A–P) C. hirsuta wild-type (A–D), par (E–H), chas1-1 (I–L), and chas1-1;par (M–P). For each genotype, rosette leaf 5 (A,E,I,M) and scanning electron micrographs of the terminal leaflet (B,F,J,N), lateral leaflet (C,G,K,O), and epidermal cells on the adaxial surface of the leaf petiole (D,H,L,P) are shown. (Q) Quantification of lateral leaflet number (primary, secondary, and tertiary) on leaf 8 of plants with the indicated genotype. n ≥ 15. (R) ChBP transcript level in C. hirsuta wild-type and par, chas1-1, and chas1-1;par. n = 3. (S–V) Rosette leaf 5 (S), terminal leaflet (T), lateral leaflet (U), and leaf petiole adaxial epidermal cells (V) of the chas1-1;par;chbp-1 mutant. Bars: A,E,I,M,S, 1 cm; B,F,J,N,T, 500 µm; C,G,K,O,U, 100 µm; D,H,L,P,V, 20 µm. Error bars in Q and R indicate standard deviation. The asterisks in Q and R indicate statistically significant difference from wild type (P ≤ 0.05, Student's t-test).