Table 3.
Productivity analysis of EMS mutagenized and dual mutagenized (EMS + UV radiation) strains. Total FAME (% of biomass) and Fatty acid profile relates to sampling at mid exponential phase. Data expressed as means ± standard deviation (n = 4).
| Exponential growth rate relative to WT (%) | Max cell density (cell/mL) | Total FAME (%) | Max. FAME productivity (μg mL−1 day−1) | Fatty acid profile |
|||
|---|---|---|---|---|---|---|---|
| SUFA | MUFA | PUFA | |||||
| WT | 100.0 | 1.1.E + 08 | 17.5 ± 0.3 | 44.5 | 39.0 ± 0.2 | 35.0 ± 0.1 | 26.0 ± 0.1 |
| NBF6-7 | 57.8 | 5.3.E + 07 | 26.4 ± 1.1 | 33.3 | 49.6 ± 1.8 | 45.2 ± 1.2 | 5.1 ± 0.6 |
| NBF6-7UV30 | 5.1 | 6.9.E + 07 | 57.3 ± 5.1 | 35.2 | 40.0 ± 4.3 | 44.0 ± 5.9 | 16.0 ± 1.6 |
| NBF7-10 | 81.8 | 4.9.E + 07 | 34.1 ± 0.8 | 49.0 | 47.8 ± 0.3 | 40.7 ± 0.0 | 11.5 ± 0.3 |
| NBF7-10UV5 | 44.2 | 8.9.E + 07 | 78.7 ± 6.2 | 37.1 | 47.0 ± 4.2 | 34.0 ± 5.8 | 19.0 ± 1.6 |