Figure 3. Histogram plots of CXCL8 monomer binding to heparin oligosaccharides.

CSP profiles of CXCL8 monomer binding to heparin dp14 (A), dp8 (B), dp4 (C) and dp2 (D). Basic residues lysine, arginine and histidine are shown as grey bars and buried residues (ASA < 40%) are shown in black. Residues showing CSPs higher than the threshold (indicated by the dotted lines) are considered as involved in binding. Lys²⁰ broadens out during the titration and is indicated by *. The CSP of His¹⁸ is truncated except for dp2 and actual CSPs are 0.39, 0.43 and 0.36 ppm for dp14, dp8 and dp4 respectively.