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. 2015 Sep 30;25(1):18–26. doi: 10.1089/scd.2014.0551

FIG. 4.

FIG. 4.

MAdCAM-1 expression on BM ECs increases in response to lethal irradiation and blocking MAdCAM-1 reduces the survival of irradiated recipients due to decreased BM homing. (A) Representative profile of flow cytometry analyses of MAdCAM-1 expression on Ter119−CD45−CD31+ BM endothelial cells (ECs) at the indicated times after total body irradiation (TBI). (B) Frequency of MAdCAM-1-expressing cells among BM ECs at the indicated times after TBI. Data are mean ± SEM (n = 4 per group, representative of three independent experiments, *P < 0.05). (C) Concentration of cytokines and chemokines in the BM supernatant (left) and peripheral blood plasma (right) at the indicated times after TBI. Samples were run in duplicate. Data are mean ± SEM (n = 2–4 per group, *P < 0.05). (D) Experimental design of the transplantation assay and in vivo homing assay using a control or MAdCAM-1 blocking antibody. (E) Survival of lethally irradiated recipients treated with control antibody or MAdCAM-1-blocking antibody before HSC transplantation (n = 10 per group, from two independent experiments, *P < 0.05). (F) Frequency of CFSE+-transplanted cells that were recovered in the BM of recipient mice at 16 h after transplantation. Data are mean ± SEM (n = 5–8 per group, from three independent experiments, *P < 0.05).