FIG. 1.

Inhibition of DC-SIGN extracellular domain (DC-SIGN ECD) binding to immobilized mannosylated-bovine serum albumin (BSA) using surface plasmon resonance (SPR). (A) DC-SIGN ECD (20 μM) was coinjected with D66 at an increasing concentration from 0 to 704 μM onto the mannosylated-BSA functionalized BIA 3000 chip surface (1847 RU). Black arrow Req represents the maximal binding response at equilibrium. The regeneration arrow corresponds to the EDTA injection for surface regeneration between each cycle. (B) Comparison of the inhibitory potency of l-fucose (▴), D66 (□), D06 (○), and d-mannose (●) toward the DC-SIGN ECD mannosylated-BSA interaction determined by competition assays. IC₅₀ values were inserted in Table 1.