FIG. 2.

TGFβ1 modulation on the expression of WNT5B in hMSC. (A) TGFβ1-induced upregulation of the expression of mRNA coding for WNT5B occurred in a dose–response manner in three different hMSC lines examined (MIO-M7, MIO-M8, and MIO-M1). Histograms represent the mean ± SEM from UV spectrophotometer readings of gel bands. Representative bands are shown below the histograms; n = 4. ANOVA test, *P < 0.05; **P < 0.01; ***P < 0.001. (B) Western blot analysis of cell lysates from hMSC showed a marked increase of intracellular protein in cells treated with TGFβ1 at a concentration of 50 ng/mL as compared with control cells. Histograms represent the mean ± SEM of the relative optical density readings of gel bands. Representative bands are shown above the histograms; n = 4. Student's t-test, *P < 0.05. (C) Minimally detectable levels of secreted WNT5B, as examined by ELISA, were observed in supernatants of cells cultured in the presence or absence of TGFβ1, and no differences between the two conditions were observed; n = 3. ns, not significant.