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. 2015 Oct 11;25(1):1–12. doi: 10.1089/scd.2015.0262

FIG. 6.

FIG. 6.

Effect of TGFβ1 inhibitors on the expression of the Wnt signaling ligands WNT2B and WNT5B by hMSC. (A) Addition of the TGFβ type I receptor (ALK5) inhibitor SB431542 (10 μM) to cells cultured with TGFβ1 antagonized the inhibitory effects of this cytokine on WNT2B mRNA expression; n = 5. ANOVA test; *P < 0.05; **P < 0.01. In contrast, addition of the JNK inhibitor SP600125 (20 μM) to cells cultured in the presence of TGFβ1 did not modify the effect of this cytokine on WNT2B gene expression. Histograms represent the mean ± SEM from UV spectrophotometer readings of gel bands. Representative bands are shown above the histograms; n = 3. ANOVA test, **P < 0.01. (B) While the ALK5 inhibitor SB431542 antagonized the upregulation of WNT5B mRNA by TGFβ1; n = 3. ANOVA test; *P < 0.05; **P < 0.01, the JNK inhibitor SP600125 did not modify the effects of this cytokine on the expression of this ligand gene; n = 4. ANOVA test, **P < 0.01; ns, not significant.