Table 1.

Primer Sequences Used for RT-PCR to Assess Gene Expression of Components of the Wnt Signaling Pathway and Photoreceptor Differentiation Markers in hMSC

Gene	Accession No.	Sequence	Tm(°C)	Product size(bp)
β-ACTIN	NM_001101	(F) CATGTACGTTGCTATCCAGGC	60	250
		(R) CTCCTTAATGTCACGCACGAT
WNT2B	NM_004185.3	(F) GACGGCAGTACCTGGCATAC	58	188
		(R) CTCCTTAATGTCACGCACGAT
WNT3A	NM_033131.3	(F) AGATGGTGGTGGAGAAGCAC	58	290
		(R) GTAGCAGCACCAGTGGAACA
WNT5B	NM_032642.2	(F) TTCTGACAGACGCCAACTC	58	264
		(R) TGACTCTCCCAAAGACAGATG
WNT8B	NM_003393.3	(F) CCATGAACCTGCACAACAA	58	174
		(R) TGAGTGCTGCGTGGTACTTC
WNT11	NM_004626.2	(F) TGACCTCAAGACCCGATACC	58	214
		(R) GCTTCCGTTGGATGTCTTGT
FZD1	NM_003505.1	(F) AGACCGAGTGGTGTGTAATGA	60	253
		(R) ATACTGTGAGTTGGCTTCGAT
FZD4	NM_012193	(F) AACTTTCACACCGCTCATC	55	391
		(R) CAGCATCATAGCCACACTTG
FZD5	NM_003468.3	(F) TTCTGGATAGGCCTGTGGTC	60	214
		(R) CGTAGTGGATGTGGTTGTGC
FZD7	NM_003507	(F) GCTCTTTACCGTTCTCACCTA	55	388
		(R) CAGGATAGTGATGGTCTTGAC
β-CATENIN	NM_001904.3	(F) TACCTCCCAAGTCCTGTATGAG	60	180
		(R) TGAGCAGCATCAAACTGTGTAG
DKK1	NM_012242.2	(F) CCTTGAACTCGGTTCTCAATTCC	60	138
		(R) CAATGGTCTGGTACTTATTCCCG
RHODOPSIN	NM_000539	(F) GCTTCCCCATCAACTTCCTCA	60	156
		(R) AGTATCCATGCAGAGAGGTGTAG
NR2E3	NM_014249.3	(F) GGCGTGGAGTGAACTCTTTC	58	230
		(R) CTGGCTTGAAGAGGACCAAG
RECOVERIN	NM_002903	(F) AGCTCCTTCCAGACGATGAA	60	150
		(R) CAAACTGGATCAGTCGCAGA

hMSC, human Müller stem cells; RT-PCR, reverse transcription-polymerase chain reaction.