Skip to main content
. 2015 Dec 20;23(18):1389–1409. doi: 10.1089/ars.2014.6221

FIG. 6.

FIG. 6.

Protein oxidation and mitochondrial function in VSMCs from young and aged mice and mitochondrial function in VSMCs from aged wild-type mice transduced with Nox4 shRNA. (A) Mitochondrial protein carbonyl content. (B) Mitochondrial OCR was determined using Seahorse XF-24 analyzer. VSMCs from 4 and 16 mo mice were plated at 25,000 cells/well in XF-24 plates and OCR was measured at the basal level and in the presence of 2 μM oligomycin (O), 500 nM Trifluoromethoxy carbonylcyanide phenylhydrazone (FCCP) (F), and 500 nM rotenone (R). (C) Mitochondrial bioenergetic parameters were derived from the measurements of OCR in VSMCs from young and aged wild-type mice. Data are the mean±SEM, n=6 (B, C). Mitochondrial complex I (D) and complex III (E) activities in VSMCs. Mitochondrial complex I (F) and complex III (G) activities in VSMCs from aged wild-type mice transduced with lentiviral scrambled and Nox4 shRNA. Data are the mean±SEM of three independent experiments (D–G). mo, month old; OCR, oxygen consumption rate.