Table 4. Pre-clinical experimental results needed to inform development of GNP-based therapies.

Experimental Validation		Key Data
In vitro	biocompatibility	GNP preparations and concentrations which are tolerated by representative normal tissues
	radiosensitization	Preliminary quantification of relationship between GNP delivery and radiosensitization to inform pre-clinical and clinical experiments
In vivo	biocompatibility	Confirmation that particles are non-toxic in concentrations intended for clinical use, and that these particles or coatings do not have unforeseen side effects.
	radiosensitization	Quantification of GNP radiosensitization in models which as closely as possible match clinical setting to confirm efficacy and inform clinical dosing and treatment planning.