FIGURE 7.

RvD1 induces M2-type phenotype and inhibits class II and co-stimulatory molecular expression in macrophages. A and B, expression of M1- and M2-specific genes, class II and co-stimulatory molecules (CD86 and CD60) were examined in adherent macrophages isolated from spleen cells of RvD1-treated and -untreated EAE groups after panning on a plastic dish and treated with lipopolysaccharide/IFNγ for 6 h (n = 4). C, SJL mice were immunized with PLP(139–151) in complete Freund's adjuvant as described before. Post-10 days, CD4 cells were isolated from spleens/lymph nodes and mixed with adherent monocytes/macrophages isolated from RvD1-treated and -untreated EAE groups at a ratio of 1:5. After 72 h of incubation, cell proliferation was examined using WST-1 reagent (n = 4). Cell supernatant was processed for IFNγ and IL17 analysis by ELISA (BioLegend) (n = 4). NS, not significant; ***, p < 0.001. D, cells were stimulated with phorbol 12-myristate 13-acetate/ionomycin for 4 h in the presence of GolgiPlug. IL17A- and IFNγ-expressing cells were measured by intracellular staining on a CD4⁺ gate (n = 2).