FIGURE 4.

Combinatorial peptide screens and sequence comparisons define a consensus motif for binding to the AP-4 β4 ear. A and B, peptides selected by GST-β4 hinge-ear (A) and GST-β4 ear (B) from a phage display library of random dodecapeptides (12-mers) fused to the N terminus of the M13 bacteriophage major coat protein. An 8-amino acid conserved core is indicated by the shaded box. C, conservation of the β4-ear-binding sequence in species from different eukaryotic super-groups. Consensus motifs for all three sequence lists are represented by sequence logos created with WebLogo.