FIGURE 6.

Alignment of conserved amino acid motifs of various bacteriocins homologous to the central domain of colicin D. Alignment of the retrieved protein sequences detected two conserved motifs, the LBS consensus sequence (column 2) and another uncharacterized consensus sequence (column 3), deduced from chromosomally encoded or plasmid-borne (p) colicin D-like ORFs aligned with Clustal-Omega Program. The consensus motifs are separated by 26 amino acids not shown, indicated by (//). Strictly or mostly conserved residues of the consensus sequences are indicated by red and black boldface type capital letters, respectively. Conserved residues in each species are highlighted for each consensus in green (LBS) and blue, respectively. The protein functions, deduced from the peptide sequence comparisons of the C-terminal domains, are indicated (column 4). Sequence references (column 1) are given under “Experimental Procedures.” ORF retrieved from P. mucilaginosus is not homologous to colicin D central domain, although it contains a well conserved LBS-like consensus motif.