TABLE 1.
Primer sequences used in quantitative PCR analysis
| Gene name | Oligonucleotide name | Primer sequence |
|---|---|---|
| Spc | q-m-Sftpc-f | Forward: 5′-CCTCGTTGTCGTGGTGAT-3′ |
| q-m-Sftpc-r | Reverse: 5′-GGTAGCGATGGTGTCTGC-3′ | |
| Podoplanin (T1α) | q-m-T1α-f | Forward: 5′-TCCACCTCAGCAACCTC-3′ |
| q-m-T1α-r | Reverse: 5′-GCTAACAAGACGCCAACTA-3′ | |
| P15 | q-m-p15-f | Forward: 5′-AGATCCCAACGCCCTGAAC-3′ |
| q-m-p15-r | Reverse: 5′-CCCATCATCATGACCTGGATT-3′ | |
| P16 | q-m-p16-f | Forward: 5′-CATCTGGAGCAGCATGGAGTC-3′ |
| q-m-p16-r | Reverse: 5′-GGGTACGACCGAAAGAGTTCG-3′ | |
| P21 | q-m-p21-f | Forward: 5′-CCAATCCTGGTGATGTCCG-3′ |
| q-m-p21-r | Reverse: 5′-TCAAAGTTCCACCGTTCTCG-3′ | |
| Acta2 (α-SMA) | q-m-αSMA-f | Forward: 5′-CCGAGCGTGAGATTGTCC-3′ |
| q-m-αSMA-r | Reverse: 5′-CGTCAGGCCAGTTCGTAGC-3′ | |
| Col1a1 | q-m-Col1a1-f | Forward: 5′-CCTACTCAGCCGTCTGTGCC-3′ |
| q-m-Col1a1-r | Reverse: 5′-AGCCCTCGCTTCCGTACTCG-3′ | |
| Tgfβ1 | q-m-Tgfb1-f | Forward: 5′-GAAGCAGTGCCCGAACC-3′ |
| q-m-Tgfb1-f | Reverse: 5′-AGCCACTCAGGCGTATCAG-3′ | |
| Tgfβri | q-m-Tgfbr1-f | Forward: 5′-CAGCTCCTCATCGTGTTGGT-3′ |
| q-m-Tgfbr1-r | Reverse: 5′-CAGAGGTGGCAGAAACACTG-3′ | |
| Tgfβrii | q-m-Tgfbr2-f | Forward: 5′-ATGCATCCATCCACCTAAGC-3′ |
| q-m-Tgfbr2-r | Reverse: 5′-TGTCGCAAGTGGACAGTCTC-3′ | |
| Bmpria | q-m-Bmpr1a-f | Forward: 5′-CTTCTCCAGCTGCTTTTGCT-3′ |
| q-m-Bmpr1a-r | Reverse: 5′-AACGACCCCTGCTTGAGATA-3′ | |
| Bmprib | q-m-Bmpr1b-f | Forward: 5′-TGTAAATGCCACCACCACTG-3′ |
| q-m-Bmpr1b-f | Reverse: 5′-TGACAACAGGCATTCCAGAG-3′ | |
| Bmprii | q-m-Bmpr2-f | Forward: 5′-ACCGCTTTTGCTGCTGTAGT-3′ |
| q-m-Bmpr2-r | Reverse: 5′-CAGAAACTGATGCCAAAGCA-3′ | |
| Il1α | q-m-IL1a-f | Forward: 5′-TCTGCCATTGACCATCTC-3′ |
| q-m-IL1a-r | Reverse: 5′-ATCTTCCCGTTGCTTGAC-3′ | |
| Il1β | q-m-IL1b-f | Forward: 5′-GCTGAAAGCTCTCCACCTCA-3′ |
| q-m-IL1b-r | Reverse: 5′-AGGCCACAGGTATTTTGTCG-3′ | |
| Il2 | q-m-IL2-f | Forward: 5′-AAGCTCTACAGCGGAAGCAC-3′ |
| q-m-IL2-r | Reverse: 5′-ATCCTGGGGAGTTTCAGGTT-3′ | |
| Il6 | q-m-IL6-f | Forward: 5′-GTTCTCTGGGAAATCGTGGA-3′ |
| q-m-IL6-r | Reverse: 5′-TTCTGCAAGTGCATCATCGT-3′ | |
| Il8 | q-m-IL8-f | Forward: 5′-ATCTTCGTCCGTCCCTG-3′ |
| q-m-IL8-r | Reverse: 5′-CAACAGTAGCCTTCACCC-3′ | |
| Il10 | q-m-IL10-f | Forward: 5′-CAGCCGGGAAGACAATAACT-3′ |
| q-m-IL10-r | Reverse: 5′-ATGTTGTCCAGCTGGTCCTT-3′ | |
| Tnfα | q-m-Tnfa-f | Forward: 5′-CCCCAAAGGGATGAGAAGTT-3′ |
| q-m-Tnfa-r | Reverse: 5′-CACTTGGTGGTTTGCTACGA-3′ | |
| Ccl2 | q-m-CCL2-f | Forward: 5′-CCCTGTCATGCTTCTGG-3′ |
| q-m-CCL2-r | Reverse: 5′-TCATTGGGATCATCTTGC-3′ | |
| β-Actin | q-m-β-actin-f | Forward: 5′-CAGCCTTCCTTCTTGGGTAT-3′ |
| q-m-β-actin-r | Reverse: 5′-TGGCATAGAGGTCTTTACGG-3′ |