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. 2015 Oct 30;290(52):30910–30923. doi: 10.1074/jbc.M115.673566

FIGURE 4.

FIGURE 4.

Up-regulation of HAS-mediated HA synthesis and down-regulation of HYBID mRNA expression by TGF-β1, bFGF, EGF, and PDGF-BB in HS27 cells. A, HA content in the culture media of HS27 skin fibroblasts stimulated with growth factors. Cells were cultured in the absence (Control) or presence of 1 or 10 ng/ml TGF-β1, bFGF, EGF, or PDGF-BB for 24 h. The HA concentrations in the conditioned media were quantified by ELISA. Values represent the mean ± S.D. (n = 3). Dunnett's test was used for statistical analysis. ***, p < 0.001; **, p < 0.01; *, p < 0.05. B and C, the expression levels of HAS2 (B) and HYBID (C) mRNAs in cells treated without (Control) or with 1 or 10 ng/ml TGF-β1, bFGF, EGF, or PDGF-BB for 8 or 24 h. Levels of mRNAs were measured by quantitative real-time PCR. HAS1 mRNA expression was negligible under either basal or stimulated conditions with the growth factors tested. Values are expressed as the mean ± S.D. (n = 3) and shown as -fold increases in mRNA expression relative to 8 h control cells. Dunnett's test was used for statistical analysis. ***, p < 0.001.