Fbw7-mediated degradation of Runx2 negatively impacts its transactivation capacity.
a, 293T cells were transfected with p6OSE2-Luc reporter vector with increasing amount of expression plasmids for Runx2 either alone or together with Fbw7α and its deletion mutants (Fbw7αΔF or Fbw7αWD). 24 h after transfection, cells were lysed, and luciferase activity was measured. b, MC3T3-E1 cells were transfected with p6OSE2-Luc plasmid and the indicated amount of expression plasmids for Runx2 either alone or together with Fbw7α and its deletion mutants as indicated. After 48 h of transfection, cells were lysed, and luciferase activity was measured. c, MC3T3-E1 cells were transfected with p6OSE2-Luc plasmid either with siFbw7 (25 and 50 nm) or with Fbw7α (0.1 and 0.2 μg). 48 h after transfection, cells were lysed, and luciferase activity was measured. Data are representative of minimum three independent experiments. Results are given as ±S.E.; *, p < 0.05, **, p < 0.001, ***, p < 0.0001. One-way analysis of variance with Tukey's multiple comparison test was performed using GraphPad Prism Version 5.00.