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Effect of TCA and S1P on S1PR2 internalization. HEK293 cells were transfected with 3×HA-tagged human S1PR2 as described under “Experimental Procedures.” Cells were stimulated with vehicle control or TCA (100 μm) or S1P (100 nm) for 10 min, the internalization process was stopped by fixing with 3.7% formaldehyde at 4 °C. The localization of S1PR2 was monitored by immunofluorescence staining. Representative images of each treatment group are shown.
