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. 2015 Oct 30;290(52):30988–31002. doi: 10.1074/jbc.M115.668277

FIGURE 9.

FIGURE 9.

The effect of TCA on EGFR transactivation in HuCCT1 cells. HuCCT1 cells were cultured in serum-free medium overnight and then treated with either 100 μm TCA for different time periods (0, 0.17, 0.5, 1, 2, 4, or 8 h), or with different concentrations of TCA (0, 25, 50, 100, or 200 μm) for 15 min. At the end of treatment, total protein was isolated. Protein levels of phosphorylated EGFR (p-EGFR) and total EGFR (t-EGFR) were detected by Western blot analysis. A and C, representative images of the immunoblots for p-EGFR and t-EGFR are shown. B and D, relative protein levels of p-EGFR/t-EGFR were calculated from three independent experiments. Values represent the mean ± S.E. of three independent experiments. Statistical significance relative to the vehicle control: *, p < 0.05; **, p < 0.01; ***, p < 0.001; relative to S1P or TCA treatment group: ##, p < 0.01; ###, p < 0.001.