FIGURE 2.

Schematic diagram for the Mgea5 promoter fragments spanning from −1034 to +181 bp. Mgea5/MGEA5 is the official name of the gene encoding the OGA protein. A, the 5′-truncated DNA fragments were cloned upstream of the firefly luciferase (LUC) reporter gene in the pGL3-basic vector. B, the truncated fragments were transfected in HEK293 and H9c2 cells, and the luciferase activity was measured 24 h post-transfection. The results are presented as relative luciferase units (RLU) (firefly luciferase/Renilla luciferase). Significant promoter activity was observed from −48 to +181 bp. All data are presented as the mean ± S.D. (error bars) from triplicate measurements (p < 0.005). C, phylogenetic conservation analysis of the human MGEA5 promoter (around 1000 bp) using the UCSC Genome Browser (version hg19) showed that the region around −300 to +600 bp of MGEA5 is highly conserved among vertebrate species. Positive peaks indicate conserved bases, and the height of the peak denotes the level of conservation. Dotted line and arrow, conserved region and gene orientation, respectively.