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. 2015 Nov 2;290(52):31013–31024. doi: 10.1074/jbc.M115.677534

FIGURE 5.

FIGURE 5.

E2F1 suppresses OGT and OGA (MGEA5) expression. E2F1 was overexpressed in HEK293 cells, and the expression of OGT and MGEA5 mRNA and protein levels were measured. A, increased E2F1 expression was observed upon transfecting the pCMV-E2F1 expression plasmid. B, qPCR analysis showed that ectopic expression of E2F1 significantly decreased OGT and MGEA5 mRNA level after 12 h of transfection (n = 4; p < 0.005). C and D, Western blot analysis (IB) showed a significant decrease in OGT (n = 4; p < 0.01) and OGA (n = 4; p < 0.05) protein level after a 48-h transfection. E, proteins from E2F1-overexpressing and control HEK293 cells were probed for O-GlcNAc Western blot that showed the same level of O-GlcNAcylation. Error bars, S.D.