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. 2015 Nov 2;290(52):31013–31024. doi: 10.1074/jbc.M115.677534

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 8. — E2F1 did not suppress OGT and OGA expression in the absence of Rb1. Rb1^−/− MEFs were transfected with E2F1 and analyzed for the expression of Ogt and Mgea5. A, Western blot analysis (IB) showed significant expression of E2F1 expression in Rb1^−/− MEFs (n = 4; p < 0.0001). B–D, Ogt and Mgea5 mRNA (n = 4) and protein levels (n = 3) were not changed upon E2F1 expression in Rb1^−/− cells compared with control. E, proteins from E2F1-overexpressing and control Rb1^−/− cells were probed for O-GlcNAc Western blot that showed the same level of O-GlcNAcylation. Error bars, S.D. *, p < 0.05.