TABLE 1.

Primers and their sequences used to clone the truncated 5′ regions of Ogt and Mgea5 promoters, ChIP assay, and quantitative PCR analysis

Primers	Sequences
Cloning primers
OGT-TPF1 (−1419 to +233 bp)	5′-ACGCACTAATCAAGAAGGCC-3′
OGT-TPF2 (−698 to +233 bp)	5′-GAGGAAAAAGGCATCCTCTT-3′
OGT-TPF3 (−294 to +233 bp)	5′-CGTACTTTAGCGCTTGCCAA-3′
OGT-TPF4 (−178 to +233 bp)	5′-GAGCCCTTAACCCGGAGTT-3′
OGT-TPF5 (−105 to +233 bp)	5′-CTTGGGCGGAAGGTTACGTA-3′
OGT-TPF6 (−66 to +233 bp)	5′-ACAGTCACTTGGTCGGTAGT-3′
OGT-TPR	5′-GGAGCTTCTTGAGATAGAAC-3′
MGEA5-TPF1 (−1034 to +181 bp)	5′-CCCTTACGCACACCGCACACTC-3′
MGEA5-TPF2 (−582 to +181 bp)	5′-AGAAGTATCGTCCCGGCGCCT-3′
MGEA5-TPF3 (−407 to +181 bp)	5′-AATGGTACGTCCCACCGCCG-3′
MGEA5-TPF4 (−48 to +181 bp)	5′-AGGCAGCGGCTGACAAA-3′
MGEA5-TPR	5′-TGGCTCTCCTTCTGCACCAT-3′
ChIP primers
mmuOGT-CP-F	5′-CGGTAGTCTTGTTACGCGCT-3′
mmuOGT-CP-R	5′-TCTACTGCCGCCACTACTGA-3′
mmuOGA-CP-F	5′-AAAATGGTACGTCCCACCG-3′
mmuOGA-CP-R	5′-GCTTCCTGTTTATCCGCACT-3′
huOGT-CP-F	5′-AAAACGTATCCGTCCCGTGT-3′
huOGT-CP-R	5′-TGTTACAATCAAGTGCCCGC-3′
huMGEA5-CP-F	5′-GTAATGGTCCGTCCGTCCC-3′
huMGEA5-CP-R	5′-CGCTTCCTGTTTATCCGCACT-3′
qPCR primers
OGT-F	5′-CCTGGGTCGCTTGGAAGA-3′
OGT-R	5′-TGGTTGCGTCTCAATTGCTTT-3′
MGEA5-F	5′-CAAGTTGCACACAGTGGAGCTAA-3′
MGEA5-R	5′-AAAGAGGGTGCAGCAACTAAGG-3′
18S-F	5′-CGAACGTCTGCCCTATCAACTT-3′
18S-R	5′-ACCCGTGGTCACCATGGT-3′