TABLE 2.

Primers and their sequences used to mutate the E2F binding sites in the Ogt (−178 bp/+233 bp) and Oga (−407 bp/+181 bp) reporter plasmids

Primers	Sequences
OGT-E2F mut-F1	5′-CGGTCGAGGAGCCCTCGATCCCATTTCAAGACAGTGA-3′
OGT-E2F mut-R1	5′-TCACTGTCTTGAAATGGGATCGAGGGCTCCTCGACCG-3′
OGT-E2F mut-F2	5′-AGTCACTTGGTCGGTAGTCTTGTTAATCGCTACCTCCGCGG-3′
OGT-E2F mut-R2	5′-CCGCGGAGGTAGCCATTAACAAGACTACCGACCAAGTGACT-3′
OGT-E2F mut-F3	5′-AGTCATGTTTGTCGCAATGGACCTTATCCGGAAGGTTACGTAAGAG-3′
OGT-E2F mut-R3	5′-CTCTTACGTAACCTTCCGGATAAGGTCCATTGCGACAAACATGACT-3′
OGT-E2F mut-F4	5′-CATGCGCTCTCTACAGTCATGATCGATCCAATGGACCTTATCCGGAAGG-3′
OGT-E2F mut-R4	5′-CCTTCCGGATAAGGTCCATTGGATCGATCATGACTGTAGAGAGCGCATG-3′
MGEA5-E2F mut-F1	5′-GCAGGGCTCCGGGCCTTCATCCCAACGGACCGAGG-3′
MGEA5-E2F mut-R1	5′-CCTCGGTCCGTTGGGATGAAGGCCCGGAGCCCTGC-3′
MGEA5-E2F mut-F2	5′-GAGCCCCGGAGGCATCCGTTCGGAGCCGC-3′
MGEA5-E2F mut-R2	5′-GCGGCTCCGAACGGATGCCTCCGGGGCTC-3′
MGEA5-E2F mut-F3	5′-GGAGGACGAGCGAAGCTACCTCCTCGGTCCTCC-3′
MGEA5-E2F mut-R3	5′-GGAGGACCGAGGAGGTAGCTTCGCTCGTCCTCC-3′
MGEA5-E2F mut-F4	5′-GCAATGGGCTTATCCCAAGGAAAAAAGGGGGAGCCGGG-3′
MGEA5-E2F mut-R4	5′-CCCGGCTCCCCCTTTTTTCCTTGGGATAAGCCCATTGC-3′
MGEA5-E2F mut-F5	5′-GTGTTCACGGCGCCCTTTGTCCTTAAACTCCCCTAGCAAATAAAG-3′
MGEA5-E2F mut-R5	5′-CTTTATTTGCTAGGGGAGTTTAAGGACAAAGGGCGCCGTGAACAC-3′
MGEA5-E2F mut-F6	5′-ACCTCCGCGGTGTTCACGATCCCCTTTGTCCTTTTTCTC-3′
MGEA5-E2F mut-R6	5′-GAGAAAAAGGACAAAGGGGATCGTGAACACCGCGGAGGT-3′