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Tertiary structure differences between the oxidized and reduced forms of Rv2466c analyzed by CD spectroscopy. A, near-UV CD spectra for Rv2466cRED and Rv2466cOX. B, thermal unfolding transitions of Rv2466cRED and Rv2466cOX monitored by the change in CD signal at 222 nm. The Tm values are given in the figures and represent the apparent melting temperature. The transition was tentatively fitted according to a two-state equilibrium (solid lines) and normalized.
