Figure 6. Molecular details of the interaction between N332 proximal glycans and PGT121 family members.

(A) Interaction of N137 and the “open face” of the members of the PGT121 family. The glycan, in red, is modeled from the PGT121 structure crystalized with a complex glycan in the open face. The evolution of critical residues (in stick-and ball representation) throughout affinity maturation of the PGT122 and PGT124 branches is highlighted in enlarged boxes. The role each residue plays in the glycan interaction at different stages in the affinity maturation is highlighted to the right of each enlarged images. (B) Interaction between the N301 glycan (in yellow ball-and-stick) and the light-chain FR3 (cartoon representation). (C) The N156 glycan (in blue ball-and-stick), represented only as its Man₃ form.