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. 2015 Nov 23;109(1):141–150. doi: 10.1093/cvr/cvv250

Figure 1.

Figure 1

Pim1 interacts with eNOS. (A) and (B) Cos7 cells were co-transfected with Flag-Pim1 and eNOS plasmids. 48 h after co-transfection, immunoprecipitation was performed with either anti-eNOS antibody (A) or anti-Flag antibody (B) to detect the interaction of eNOS with Pim1. (C) and (D) Endogenous eNOS interacts with Pim1 in HUVECs, as determined by immunoprecipitation with anti-eNOS antibody (C) and anti-Pim1 antibody (D), respectively. All experiments were repeated three times.