Fig. 1. Synergistic cytotoxicity between doxorubicin and lipid-loading in Huh7 cells. (A) Huh7 cells were exposed to varying concentrations of fatty acids or a 300 μM FFA mixture (2 : 1 v/v oleate : palimitate) for 24 h and then cell viability measured by MTT assay. Huh7 cells, either naïve (black bars) or pre-loaded with 300 μM FFA mixture for 24 h (open bars), were exposed to varying concentrations of fatty acids, or doxorubicin for 24 h, and then cell viability (B) or (C) caspase 3/7 activity was measured. A combination index was calculated to examine potential interactions between the FFA mixture and doxorubicin. Each data point represents the mean of three independent experiments. Error bars represent the standard error of the mean (SEM). * = p < 0.05, ** = p < 0.01, *** = p < 0.001 compared to vehicle control or the indicated comparison.