Fig. 2. Additive lipid-accumulation between doxorubicin and lipid-loading in Huh7 cells. Huh7 cells, either naïve or pre-loaded with 300 μM FFA mixture (2 : 1 v/v oleate : palimitate) for 24 h, were exposed to 3.6 μM doxorubicin for 24 h. Intracellular lipid accumulation was detected by Nile Red and (A) quantified or (B) visualised by fluorescence microscopy. For lipid quantitation, each data point represents the mean of three independent experiments. Error bars represent the standard error of the mean (SEM). * = p < 0.05.