Fig. 4. Synergistic oxidative stress between doxorubicin and lipid-loading in Huh7 cells. Huh7 cells, either naïve or pre-loaded with 300 μM FFA mixture (2 : 1 v/v oleate : palimitate) for 24 h, were exposed to the indicated concentration of doxorubicin for various times and then various parameters measured: (A) ROS, (B) transcripts associated with oxidative stress response, and (C) metallothionein protein levels. Each data point represents the mean of three independent experiments. Error bars represent the standard error of the mean (SEM) * = p < 0.05, ** = p < 0.01, *** = p < 0.001.