| Reagent | Volume in mix (µl) |
| Protein | To final concentration of 0.11 mg/ml |
| 5000X SYPRO Orange | 0.3 |
| 0.5 M HEPES pH 7.0 | 3.7 |
| 5 M NaCl | 5.6 |
| Water | To 180 µl |
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