Reagent | Volume in mix (µl) |
Protein | To final concentration of 0.11 mg/ml |
5000X SYPRO Orange | 0.3 |
0.5 M HEPES pH 7.0 | 3.7 |
5 M NaCl | 5.6 |
Water | To 180 µl |
Reagent | Volume in mix (µl) |
Protein | To final concentration of 0.11 mg/ml |
5000X SYPRO Orange | 0.3 |
0.5 M HEPES pH 7.0 | 3.7 |
5 M NaCl | 5.6 |
Water | To 180 µl |