Fig 4. Evaluation of protective efficacy of Cipa extract in vivo.

AG129 mice (9–12 weeks old) were injected i.p. with 10⁶ PFU brain-passaged DENV-2. Infected mice were treated orally with 0.25% methyl cellulose (solid red squares) or Cipa extract at 125 mg (empty blue circles) and 250 mg/kg body weight (solid blue circles). Treatment was twice daily for the first 5 days. A sham control group that was not virus-infected, but which received the higher dose of Cipa extract orally (solid green squares), was tested in parallel. The mice were monitored daily for mortality and the resultant data plotted as Kaplan-Meier survival curves. The p values to assess the statistical significance in the survival rates on day 35 between the Cipa extract-treated and placebo-treated (0.25% methyl cellulose) groups were determined using the Log-rank test.