Fig 5. Homing defects of Rras ^−/− T cells.

(A) Rras ^+/+ (WT) and Rras ^−/− (KO) naïve T cells were separately labeled with PKH26 and CFSE, respectively. Then, 5 × 10⁶ cells of each population were co-mixed and injected into WT mice. After 2 h, the uninjected cell mix (Before), lymph nodes (LN), and spleen (Spl) were analyzed with flow cytometry. (B) The relative ratios of Rras ^+/+ to Rras ^−/− T cells are depicted. Results are from a representative experiment and was reproduced twice. Bars, SD. Paired t-test. *p<0.05, **p<0.005. (C) CFSE-labeled T cells were injected into WT mice, and after 6 days, different lymphocyte subsets in PLNs were quantified by flow cytometry. Bars, SE. *p<0.05, ns, not significant.